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KMID : 0613820090190050639
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Journal of Life Science
2009 Volume.19 No. 5 p.639 ~ p.643
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Development of DNA Markers for Trehalose Synthesis Genes in Brassica rapa L.
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Jeong Ye-sol
Lim Yong-Pyo
Hur Yoon-Kang
Chung Sang-Min
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Abstract
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High temperature stress might affect the yield and quality of Chinese cabbage. In order to develop cultivars resistant to high temperature stress, we developed polymorphic DNA markers for trehalose synthesis genes related to abiotic stress resistance. A total of 28 Brassica rapa ESTs homologous to trehalose synthesis genes of Arabidopsis were found from the NCBI database. The polymorphic DNA sequences were searched between Chinese cabbages - Chiifu, which is relatively susceptible to high temperature stress, and Kenshin, which is tolerant to high temperature stress. Among the 28 ESTs, we found 10 ESTs that have either insertion/deletion and/or single nucleotide polymorphism between the two cultivars. Those polymorphic sites were then targeted for the development of 10 PCR based markers. These molecular markers related to trehalose genes could be used not only to test their relationship with abiotic stress resistance in Chinese cabbage, but also the development of abiotic stress resistant cultivars using MAS.
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KEYWORD
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Chinese cabbage, abiotic stress, trehalose, molecular marker
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